2. Put the females put in separate pill boxes, give the box a lot number, and place the box over moist sand so they can lay eggs.
Div
class
grid-col-6
In this undated photograph, students pose with their teachers on the steps of the Canyon Creek Schoolhouse in Montana. In the fall of 1921, students would have to cross the bridge over the Bitterroot River to attend school in the town of Hamilton, abandoning their old brick school.
Image: Office of NIH History and Stetten Museum, 1487-1
Div
class
grid-row grid-gap
Div
class
grid-col-6
3. After the eggs hatch, feed the whole group of larvae on infected rabbit. Do the same after they molt and become nymphs (as shown in the tubes) on a non-infected rabbit.
4. After each feeding, inject a few ticks into a guinea pig’s abdomen to see if the guinea pig gets RMSF. Then you know the ticks are infected too.
5. After the ticks molt to become adults, keep them a month to let the RMSF infection grow in them.
Div
class
grid-col-6
In this undated photograph, students pose with their teachers on the steps of the Canyon Creek Schoolhouse in Montana. In the fall of 1921, students would have to cross the bridge over the Bitterroot River to attend school in the town of Hamilton, abandoning their old brick school.
Image: Office of NIH History and Stetten Museum, 1486
