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12. Bottle the vaccine in a sterile environment as this worker is doing in this 1931 photo.

The dosage is difficult to determine, but “Such irregularities are not surprising, however, when we recall that so little is known of the various factors affecting the process and mechanism of immunity.”

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A woman bottles vaccine under a glass enclosure. She wears an apron but no protective gloves or mask.

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Image: Office of NIH History and Stetten Museum, 1523-sl

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