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After the administrative transfer of the DBS from the NIH to the Food and Drug Administration (FDA) in 1972, Barker moved to the Division of Blood and Blood Products where he worked from 1973 to 1978. Barker researched the hepatitis B virus and its test and vaccine. He then worked at the American Red Cross from 1978 to 1991.

Barker returned to the NIH in 1991, working at the National Institute of Allergy and Infectious Diseases (NIAID) in the Division of AIDS. Since After retiring from NIH in 1997, he has worked at a pharmaceutical company and served as a medical consultant to several organizations. 

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