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The FLICKER software allowed users to measure spots as a set of (X,Y) image positions and integrated-densities for each sample. We manually recorded this data for later analysis, manually defining spots to be corresponding if they aligned well with FLICKER.

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Figure 18. This was the alternative image acquisition setup. (Reproduced from a figure with permission from a reprint from Environmental Health Perspectives, 1980 [9].) A high-quality uniform illumination Aristo light box and Quantimet Vidicon TV camera were used for non-microscope images. A gel autoradiograph, wet 2D gel, electron micrograph, or other transparent sample object was placed on the light box and scanned using the RTPP/Vidicon camera. A NBS standard neutral density step wedge was placed on the bottom of the scan area. This was captured along with the image and was used to calibrate the grayscale pixel data to optical density using a piecewise linear curve fitting algorithm (see [9] for an example). As an aside, the electronics workbench area in the background was where many of the local RTPP electronics assembly was performed in the Park Building.

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