Staphylococcal nuclease was the focus of extensive experimental work in Christian Anfinsen’s Laboratory of Chemical Biology from 1963 to 1975. During this period, it was one of the best understood proteins and was the basis for the formulation of a number of new approaches and concepts in the area of protein structure and function.

Molecular models were built as part of Protein Chemistry seminars conducted for NIH Research Associates in the 1960s by Anfinsen and David Davies. Digital graphics and 3-D printed models have largely supplanted hand-built models.

Only ten years after publishing The Molecular Basis of Evolution, Anfinsen was able to use protein chemistry to investigate what happens when a gene mutates and causes a protein to change. Anfinsen stated in a 1970 interview in the Jerusalem Post that “[W]e are engaged in what you may call molecular engineering. We look at the structure of an enzyme, for instance, and if we see a loop in the chain that doesn't seem to be doing anything, we see what happens if we chop it off. Some people seem to think that the most important discoveries have already been made in molecular biology, that the double helix wrapped it up, but I think that [protein folding] just opened up an enormous field for us.”

Learn More about this molecule on the Staphylococcal Nuclease Model Artifacts page.

Image Added

3D-printed Staphylococcal nuclease

NIH 3D Print Exchange Model ID 3DPX-000590 by Reid Alderson.

Model 3D printing and finishing courtesy of James Tyrwhitt-Drake, Medical Science and Computing, Inc., Bioinformatics and Computational Biosciences Branch, NIH/NIAID/OD/OSMO/OCICB.